1. What is Centrifuge G Force?

G force (relative centrifugal force) is a measurement of the acceleration applied to a sample in a centrifuge. It is expressed as a multiple of the earth's gravitational acceleration (g).

2. How Does the Calculator Work?

The calculator uses the centrifuge G force equation:

Where:

• \( G \) — Relative centrifugal force (g)
• \( RPM \) — Rotations per minute
• \( r \) — Rotor radius in centimeters
• \( 1.118 \times 10^{-5} \) — Conversion factor

Explanation: The equation converts rotational speed (RPM) to acceleration by accounting for the radius of rotation.

3. Importance of G Force Calculation

Details: Accurate G force calculation is crucial for proper centrifugation protocols, ensuring consistent results across different centrifuges and rotors.

4. Using the Calculator

Tips: Enter RPM (rotations per minute) and rotor radius in centimeters. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: Why is G force important in centrifugation?
A: G force determines the sedimentation rate of particles. Different protocols require specific G forces for optimal separation.

Q2: How does rotor radius affect G force?
A: At the same RPM, a larger rotor radius produces higher G force due to greater centrifugal acceleration.

Q3: What are typical G forces used in laboratories?
A: Common ranges: low speed (1000-5000 g), high speed (10000-30000 g), ultracentrifuges (50000-100000+ g).

Q4: Should I use maximum radius or average radius?
A: For most calculations, use the maximum radius (distance from center to bottom of tube).

Q5: How does G force relate to sedimentation time?
A: Higher G forces reduce sedimentation time. Doubling G force halves the time needed for equivalent sedimentation.